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After the last wash, all liquid was carefully removed, the beads were resuspended in 35 l of 1x Laemmli buffer and boiled for 1 min

Chura-Chambi RM, Farah CS, Morganti L (2022) Human growth hormone inclusion bodies present native-like secondary and tertiary structures which can be preserved by mild solubilization for refolding

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Another study of older adults found almost all had at least one PVC a day, and that the typical rate was about two per hour